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New England Biolabs
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Journal: bioRxiv
Article Title: Optimising the use of Oxford nanopore sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes
doi: 10.1101/2025.06.16.659891
Figure Lengend Snippet:
Article Snippet: Extracted gDNA from individual mosquitoes was prepared by diluting 10ng of DNA in 15μL of nuclease free water and then the diluted DNA was combined with
Techniques: Sequencing
Journal: bioRxiv
Article Title: Optimising the use of Oxford nanopore sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes
doi: 10.1101/2025.06.16.659891
Figure Lengend Snippet: All bar graphs depict sequencing runs using two different barcoding kits: the Rapid PCR Barcoding Kit 24 V14 (runs 2-6) and the 16S Barcoding Kit 24 V14 (runs 7,8,9,11,12). Each run is represented by a vertical column. A ) Total Data generated (GB). B ) Percentage of passed vs failed bases called for each run. C ) Total number of reads obtained in each sequencing run. D ) Percentage of unclassified passed reads and unclassified passed bases for each run.
Article Snippet: Extracted gDNA from individual mosquitoes was prepared by diluting 10ng of DNA in 15μL of nuclease free water and then the diluted DNA was combined with
Techniques: Sequencing, Generated
Journal: bioRxiv
Article Title: Optimising the use of Oxford nanopore sequencing technology for detection of Wolbachia bacterial endosymbionts in Anopheles mosquitoes
doi: 10.1101/2025.06.16.659891
Figure Lengend Snippet: Libraries prepared and sequenced using either the Rapid PCR Barcoding Kit 24 V14 (Rapid) or 16S Barcoding Kit 24 V14 (16S).
Article Snippet: Extracted gDNA from individual mosquitoes was prepared by diluting 10ng of DNA in 15μL of nuclease free water and then the diluted DNA was combined with
Techniques:
Journal: Frontiers in Immunology
Article Title: The gut-brain-axis one year after treatment with cladribine tablets in patients with relapsing remitting multiple sclerosis: a pilot study
doi: 10.3389/fimmu.2025.1514762
Figure Lengend Snippet: Antibodies for CyTOF.
Article Snippet: We first barcoded the samples using
Techniques:
Journal: Frontiers in Immunology
Article Title: The gut-brain-axis one year after treatment with cladribine tablets in patients with relapsing remitting multiple sclerosis: a pilot study
doi: 10.3389/fimmu.2025.1514762
Figure Lengend Snippet: Different repopulation of various immune cells after cladribine treatment. (A) UMAP plot displaying CD45 + immune cells from the blood of cladribine-treated patients at different time points. Colors correspond to PARC-guided clustering. (B) Density UMAP plots showing the proportion of cells assigned to each time point according to each cluster. Color key indicates high (red) and low (blue) percentage of immune cells. (C) Percentage of each annotated cell population out of the total CD45 + immune cells at each time point. Each data point corresponds to each individual, colored by time point. Significant P-values are stated in the graphs. D0: baseline visit; M3: 3 month visit; M12: 12 month visit.
Article Snippet: We first barcoded the samples using
Techniques: